Figure 1.

Dengue poly‐immunoglobulin G scaffold (D‐PIGS) induce cellular and humoral immune responses in human adenoid/tonsil cultures. (a) Schematic representation of the monomeric and polymeric human IgG1‐cEDIII fusion protein and its interaction with high and low‐affinity Fc gamma receptors on antigen‐presenting cells; further details of the structure of these molecules are described in Kim et al. (2017). Red represents cEDIII domain, while dark and light blue indicate CH2 and CH3 domains of human IgG1, respectively. (b) Immunogenicity of D‐PIGS in human tonsil cultures; shown are flow cytometric data of CFSE staining expressed as percentages of antigen‐specific CD4‐ and CD8‐proliferating cells, IFN‐γ and IgG concentrations in culture supernatants, induced by cEDIII alone, monomer/single chain (M/S) and polymer (P, D‐PIGS). Data are shown as means ± SEM from eight patients. Statistical analysis was performed by ANOVA and Dunnett's test, where *indicates differences <0.05 and **<0.01.