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. 2018 Feb 1;16(7):1283–1294. doi: 10.1111/pbi.12869

Figure 3.

Figure 3

Biacore analysis of binding of dengue poly‐immunoglobulin G scaffold (D‐PIGS) to Fc gamma receptors. (a) Schematic representation of affinity vs avidity measurement. (b) Affinity measurement of the interaction between immobilized (200RU) unfractionated D‐PIGS and hIgG1 to CD64 (120, 60, 30, 15 nm) and CD16a (800, 400, 100, 25, 12.5 nm). The panel on the right indicates calculated affinity constants ( K D ) for each interaction as calculated using either the 1 : 1 Langmuir model (CD64) or the ‘two‐state reaction’ model (CD16a). (c) Binding (avidity) of unfractionated D‐PIGS and human IgG1 to his‐tag captured (immobilized) CD64, CD32a and CD16a receptors at a surface density of 200 pg/mm2.