Fig. 2.

Bacterial counts in Blood, Brain and Lungs. Fig. 2A shows Levels of bacteria in the blood, lungs and brain of vaccinated mice (preparation 1) post challenge with TIGR4. Bacteria in the blood is shown at 24 h post challenge (taken via tail vein bleed) and at time of death (TOD, taken via cardiac puncture under terminal anesthesia). No statistical analysis was performed on data, n = 3 mice/group. Fig. 2B shows levels of bacteria in the blood of vaccinated mice (preparation 1 and 2) at 24 h post challenge with TIGR4 (tail vein bleed) and at the TOD. Red circular symbol represents data from the mouse that was vaccinated with AcrA-SP4 P2 and survived challenge. Statistical analysis was performed in GraphPad Prism using a non-parametric Mann-Whitney two sample rank test, significance *P < 0.05, n = 3–5 mice/group. Each dot represents counts from a single animal.