FIGURE 4.

Analyses indicating oxidative stress in ischemic limbs of treated and nontreated diabetic and control mice 14 days after ligation. Oxidative stress levels are higher in diabetic mice than in nondiabetic mice. However, in the diabetic and nondiabetic groups, oxidative stress levels are lower in mice treated with DATS. A, Representative photographs of nitrotyrosine immunostaining; scale bar = 50 μm. B, Quantitative analysis of nitrotyrosine immunostaining. C, Protein carbonyl levels. **P < 0.01 cf. nondiabetic. ##P < 0.01 cf. diabetic.