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. Author manuscript; available in PMC: 2019 Jun 13.
Published in final edited form as: J Am Chem Soc. 2018 Jun 4;140(23):7116–7126. doi: 10.1021/jacs.8b01933

Figure 1.

Figure 1

Kinetics of the ferryl complex in NapI monitored by its absorbance at 320 nm. An anoxic solution of NapI (1.2 mM), Fe(II) (1.1 mM), 2OG (6.0 mM) and 6.0 mM of either L-Arg (black), 4,4-d2-L-Arg (red), or 5,5-d2-L-Arg (blue) was mixed at 5 °C with an equal volume of air-saturated buffer (giving ~ 0.19 mM final [O2]). Regression fits to the data are shown as solid lines. The equation for fitting assumes absorbance from two consecutive, irreversible reactions, with isosbestic reactant and product and more intensely absorbing intermediate.