Table 2.
Reagents details
Antibodies used for immunocytochemistry/flow-citometry | |||
---|---|---|---|
Antibody | Dilution | Company Cat # and RRID | |
Pluripotency Markers | Rabbit anti-SOX2 | 1:100 | Santa Cruz Biotechnology Cat# sc20088, RRID: AB_2255358 |
Pluripotency Markers | Rabbit anti-NANOG | 1:100 | PeproTech Cat# 500-P236, RRID: AB_1268274 |
Pluripotency Markers | Rabbit anti-OCT4 | 1:200 | Santa Cruz Biotechnology Cat# sc9081, RRID: AB_2167703 |
Secondary antibodies | Cy2 Donkey Anti-Rabbit IgG | 1:100 | Jackson ImmunoResearch Cat# 711-225-152, RRID: AB_2340612 |
Secondary antibodies | Alexa Fluor 555 Donkey Anti-Rabbit IgG | 1:100 | Life Technologies Cat# A31572, RRID: AB_162543 |
Primers | |||
Target | Forward/Reverse primer (5′-3′) | ||
Genotyping | Dystrophin exons 44–51 | ATCTGTTGAGAAATGGCGGC/ GATGGTGGGTGACCTTGAGG |