Fig. 2. THR peptide significantly enhances AAV8 transduction in vivo, especially in the brain.

5×10¹⁰ vg of AAV8/luc alone or the complex of pre-incubated AAV8 and 0.1 mM of peptide at 4 °C for 2 h were administered via an intravenous injection. At one-week post- injection, in vivo luminescence imaging (A) was performed and the photon signal (B) was measured and calculated. At three-week post-injection, the mice were euthanized and their tissues were harvested for DNA extraction. Relative luciferase expression levels (C) and vector copy numbers (D) of different tissue lysates were determined separately. The data of each group represent the average and SD from five mice. *** p < 0.001, ** p < 0.01, and *p < 0.05 compared to the control mice with an AAV8 treatment only. The “ns” indicates no significant difference (p> 0.05).