(A) A schematic administration of the AAV8 vectors. Three groups were assigned: AAV8 only, AAV8/0.1 mM THR and AAV8-0.1 mM THR (B) Images were taken for luminescence expression on days 3, 7, 14, and 21 following the AAV8 only, AAV8/0.1 mM THR, and AAV8-0.1 mM THR after a systemic administration. (C) The average liver luciferase signal was calculated for the different groups. (D) Relative luciferase expression level and vector copy numbers in the brain of each group were determined separately. The data of each group represents the average and SD from five mice. ****p < 0.001 compared to the control mice with the AAV8 treatment only.