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. 2018 Jun 13;9(6):707. doi: 10.1038/s41419-018-0725-4

Fig. 3. HOXA-AS3 knockdown inhibits cell migration, invasion, and colony formation in A549 cells.

Fig. 3

a Left, A549 cells were subjected to a scratch-wound assay. Scale bar, 50 μm. Right, the histogram shows the cell migration ability of A549 cells. b Left, A549 cells were subjected to Matrigel® invasion chamber analyses. Scale bar, 50 μm. Right, the histogram shows the cell invasion ability of A549 cells. c Determination of the efficiency of shRNA directed against HOXA-AS3 using the quantitative polymerase chain reaction (qRT-PCR). d A colony forming growth assay was performed to determine the proliferation in A549 cells. NC, negative control; siRNA/HOXA-AS3, small interfering RNA for HOXA-AS3; shRNA/NC, small hairpin RNA for negative control; shRNA/HOXA-AS3, small hairpin RNA for HOXA-AS3. *P < 0.05, **P < 0.01, and ***P < 0.001. All values are expressed as the mean ± SEM