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. 2018 Jun 13;9(6):706. doi: 10.1038/s41419-018-0724-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The efficiency of the plasmids of linc00462 was identified by RT-qPCR assay. b CCK8 asssay showed the effect of linc00462 on cellular viabilities. c Colony formation assay showed linc00462 overexpression promoted relative colony formation rate in PC cells. d EdU incorporation assays showed that linc00462 promoted the cell numbers of EdU staining. e The cell cycle of linc00462 was detected by flow cytometry assay. f The related protein levels of cell cycle were detected by western blot assay. g Cell apoptosis assays showed that linc00462 overexpression inhibited the apoptosis by flow cytometry assay. h Hoechst/PI staining assays showed that linc00462 overexpression inhibited the apoptosis. i The related protein levels of cell apoptosis were detected by western blot assay. All results performed above are presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, NS not significant