Fig. 3. linc00462 promoted EMT and tumor growth, inhibited cell-matrix adhersion.

a, b Transwell assay showed the effect of linc00462 on cell migration and invasion ability. c Adhesion assay of PC cells transfected with the indicated plasmids at the indicated time. The number of adherent cells was estimated by reading the absorbance at 620 nm. The photomicrographs were taken at ×100 magnification. d The related protein levels of EMT process were detected by western blot assay. e, f The tumor size and weight were significantly increased after treated with lenti-linc00462. g Comparison of spontaneous metastasis and images of representative metastatic foci in the lung, liver, colorectum and spleen by HE staining. All results performed above are presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, NS not significant