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. 2018 Jun 13;9(6):706. doi: 10.1038/s41419-018-0724-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The levels of miR-665 in CFPAC-1 and PANC-1 cells were detected by RT-qPCR assay. b The efficiency of the indicated plasmids of miR-665 was identified by RT-qPCR assay. c The relationship of miR-665 and linc00462 was shown used RegRNA 2.0 and putative and mutant binding sites of miR-665 on linc00462 were shown. d EGFP intensity was measured in cells co-transfected with pri-miR-665 or ASO-miR-665 and linc00462 wt or mutant construct. e RT-qPCR assay was used to assess linc00462 levels in PC cells in response to altered miR-665 expression. f RT-qPCR assay showed the miR-665 levels when the expression of linc00462 was altered. g CCK-8 assay showed the cell viability of PC cells treated with the indicated plasmids. h Flow cytometry assay showed cell cycle of PC cells treated with the indicated plasmids. i Western blot assay showed the related protein levels of cell apoptosis treated with the indicated plasmids. j Transwell assay showed the cell migration and invasion transfected with the indicated plasmids. All results performed above are presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, NS not significant