Fig. 7. Linc00462 and miR-665 regulate the SMAD2/3 pathway.

a The indicated protein levels were detected by western blot under the non-treated condition in CFPAC-1 cells. b The indicated protein levels were detected by western blot treated with the TGFBR1/2 inhibitor SB431542 for 2 h in CFPAC-1 cells. c, d The distribution of SMAD2/3 was shown by IF assay treated with the TGFBR1/2 inhibitor SB431542 for 2 h or not in CFPAC-1 cells. e, f Relative mRNA levels of collagen 1, collagen 3, and fibronectin were detected by RT-qPCR assay treated with the TGFBR1/2 inhibitor SB431542 for 2 h or not in CFPAC-1 cells. g, h ELISA assay showed the levels of collagen 1, collagen 3, and fibronectin treated with the TGFBR1/2 inhibitor SB431542 for 2 h or not in CFPAC-1 cells. All results performed above are presented as mean ± SD from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, NS not significant