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. 2018 Jun 13;9(6):706. doi: 10.1038/s41419-018-0724-5

Table 2.

The primers used in this study

RT-qPCR primers Sequences (5′-3′)
Linc00462-qPCR-forward ACTAGGTCCTTCTGGTGTT
Linc00462-qPCR-reverse GTAAAACTTGCTGCTGATG
U6-RT GTCGTATCCAGTGCAGGGTCCGA
GGTGCACTGGATACGACAAAATATGG
U6-qPCR-forward TGCGGGTGCTCGCTTCGGCAGC
Reverse-miRNAs CCAGTGCAGGGTCCGAGGT
β-actin-qPCR-forward CGTGACATTAAGGAGAAGCTG
β-actin-qPCR-reverse CTAGAAGCATTTGCGGTGGAC
Oligo dT TTTTTTTTTTTTTTTTTT
miR-665-RT GTCGTATCCAGTGCAGGGTCCG
AGGTGCACTGGATACGACAGGGGC
miR-665-qPCR-forward TGCGGACCAGGAGGCTGAG
TGFBR2-qPCR-forward TCTGGGCTCCTGATTGCT
TGFBR2-qPCR-reverse TGAGGCAGCTTTGTAAGT
TGFBR1-qPCR-forward AAAACATTATCGCAACTCAG
TGFBR1-qPCR-reverse CACAGAAAGGACCCACAT
COI-I-qPCR-forward GGCAACAGCCGCTTCACCT
COI-I-qPCR-reverse AGGGAGCCAGGTTGGGATG
COI-III-qPCR-forward CCCTGTCTGCTTCCTGTA
COI-III-qPCR-reverse GCAACCATCCTCCAGAAC
FN-qPCR-forward TGAACCCAGTCCCGAAGG
FN-qPCR-reverse AACTCCCAGGGTGATGCT
Plasmids primers
 linc00462-S CGGGGTACCCCCAGTCTCGGATAAGTC
 linc00462-AS CCGCTCGAGGTCCTCGAACATTCCACT
 miR-665-S CGCGGATCCCTTTGTGCCCAGGGTGGA
 miR-665-AS GAATTCAGACAGAGGTCCAAGAACCAG
 TGFBR1-S CCCAAGCTTGAATCCATGGAGGCGGCGGTCGCTGCT
 TGFBR1-AS CTCGAGTTACATTTTGATGCCTTCCTGTTGA
 TGFBR2-S CCCAAGCTTGAATCCATGGGTCGGGGGCTGCTCAGG
 TGFBR2-AS CTCGAGTTA CTATTTGGTAGTGTTTAGGGAGCCG
 ASO-NC CAGUACUGUAGUGUAGUACTT
 ASO-miR-665 AGGGGCCUCAGCCUCCUGGU
 shR-linc00462-S CCGGAAGCACAGTGGTCTAAAAGTA
CTCGAGTACTTTTAGACCACTGTGCTTTTTTTG
 shR-linc00462-AS CAAAAAAAGCACAGTGGTCTAAAAGT
ACTCGAGTACTTTTAGACCACTGTGCTTCCGG
 shR-TGFBR1-S GATCCGGGCTGACAGCTTTGCGAATTAA
CTCGAGTTAATTCGCAAAGCTGTCAGCTTTTTA
 shR-TGFBR1-AS AGCTTCAAAAAGGGCTGACAGCTTTGCGAATT
AACTCGAGTTAATTCGCAAAGCTGTCAGCG
 shR-TGFBR2-S GATCCGGGACCTCAAGAGCTCCAATAT
CCTCGAGGATATTGGAGCTCTTGAGGTCTTTTTGA
 shR-TGFBR2-AS AGCTTCAAAAAGGGACCTCAAGAGCTCCA
ATATCCTCGAGGATATTGGAGCTCTTGAGGTCG