Fig. 7. Silencing of ANO1 inhibits xenograft tumor growth in nude mice by activating TNF-α signaling.

a Body weight in nude mice. The lentiviral vector pLKO.1 was used to construct ANO1 shRNA1 and control shRNA plasmids. In total, 2 × 10⁶ PC-3 cells with lentiviral infection of ANO1 shRNA1 or NCsh (negative control scrambled shRNA) were injected into the right flank of nude mice of the same age. b Knockdown of ANO1 resulted in significant inhibition of xenograft tumor growth. Tumor volumes in each group were measured with calipers once a week. The data are shown as the average tumor volume in mm³ ± SEM (NC, n = 5; shRNA1, n = 6). ***p < 0.001 vs NC. c Comparisons of tumor bearing mice and tumors that were removed on the 35th day from groups of negative control and ANO1 siRNA1. d Tumor weights in nude mice between groups of negative control (NC, 352 ± 36 mg, n = 5) and ANO1 siRNA1 (shRNA1, 63 ± 30 mg, n = 6). ***p < 0.001 vs NC. e Silencing ANO1 increased the mRNA expression of TNF-α, CASP3, and CASP7 in xenograft tumors. The relative mRNA expressions were examined using qPCR and presented after being normalized to β-actin (means ± SEM; NC, n = 5; shRNA1, n = 3. *p < 0.05; **p < 0.01 vs NC)