Fig. 2. Bta-miR-499 is highly enriched in placenta-specific exosomes.

a qPCR verification of the top ten differentially sorted miRNAs in exosomes during the non-pregnant stage (G_D.0) and early pregnancy (G_D.60) in dairy cows (n = 3). b Bta-miR-499 levels were analyzed in exosomes isolated from the plasma of non-pregnant (G_D.0, n = 5) and early pregnant cows (G_D.60, n = 5) by qPCR; U6 was used as the internal control. c The levels of bta-miR-499 in peripheral blood, early embryo and uterus during early pregnancy (G_D.60) in dairy cows (n = 3); U6 was used as the internal control. G_D.0 and G_D.60 represent gestational day 0 and 60, respectively. Data represent three independent experiments and are presented as the mean ± S.E.M (error bars). Two-tailed Student’s t-test, *P < 0.05; **P < 0.01