Fig. 6. Bta-miR-499 upregulates bta-let-7 miRNAs via targeting Lin28B.

a BEND cells were transfected with agomiR-499, and the expression of the bta-let-7 family was determined by qPCR. U6 was used as a control. b Lin28B was overexpressed using the pCDNA.3.1-Lin28B vector in BEND cells, and the expression levels of bta-let-7 family members that were decreased by agomiR-499 were detected by qPCR. U6 was used as a control. c qPCR analysis showing the effect of placental exosomes (p-EXO) and non-pregnant exosomes (n-EXO) on the expression of the bta-let-7 family; U6 was used as a control. d BEND cells were induced by LPS, and Lin28B levels were detected by western blotting. β-actin was used as a control. e BEND cells were transfected with Lin28B siRNA for 24 h and then induced by LPS for 1 h; the levels of the bta-let-7 family were determined by qPCR. U6 was used as a control. Data represent three independent experiments and are presented as the mean ± S.E.M (error bars). Two-tailed Student’s t-test, *P < 0.05; **P < 0.01