Figure 3.

Effects of U0126, LMB and roscovitine on p27^Kip1 expression in the CA1 region following SE. (A) Representative double immunofluorescent images for GFAP and p27^Kip1 expression. SE reduces nuclear p27^Kip1 expression in CA1 neurons as well as astrocytes. As compared to vehicle, LMB attenuates the decline of nuclear p27^Kip1 expression in CA1 neurons, while U0126 and roscovitine do not. However, U0126, LMB and roscovitine abrogate the decline of nuclear p27^Kip1 expression in astrocytes. Insert images are high magnification of rectangles in merge panels. Bar = 50 and 12.5 (inserts) μm. (B,C) Quantifications of the number of nuclear p27^Kip1 expression in CA1 neuron (B) and astrocytes (C). Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM (*,^#p < 0.05 vs. control and vehicle, respectively; n = 7, respectively).