FIGURE 1.

Effects of EL on the degranulation of mast cells. (A) Chemical structure of EL. (B) RBL-2H3 cells (6 × 10⁴/well) were pretreated with or without EL for 12 h and then incubated with 1 mg/mL MTT for 2 h. The absorbance intensity was measured using a spectrophotometer. (C–G) RBL-2H3 cells, BMMCs (5 × 10⁵/well), and RPMCs (2 × 10⁴/well) were sensitized with anti-DNP IgE (50 ng/mL). After overnight incubation, the cells were pretreated with or without drugs, including EL and Dexa, for 1 h or 30 min and then challenged with DNP-HSA (100 ng/mL). (H) HMC-1 cells (1 × 10⁶/well) were pre-incubated with EL for 30 min prior to incubation with PMACI for 30 min. β-Hexosaminidase and histamine levels were detected using a spectrophotometer or a fluorescent plate reader, respectively. Graph data represent the mean ± SEM of three independent experiments. ^∗p < 0.05. Dexa, dexamethasone.