FIGURE 3.

Effects of EL on the expression and secretion of inflammatory cytokines and the activation of signaling proteins and NF-κB. RBL-2H3 cells (5 × 10⁵/well) were sensitized with anti-DNP IgE (50 ng/mL). After overnight incubation, the cells were pretreated with or without drugs, including EL and Dexa, for 1 h, and then challenged with DNP-HSA (100 ng/mL). (A) The gene expression of inflammatory cytokines was determined by qPCR. (B) The secretion of inflammatory cytokines was measured by ELISA. Graph data represent the mean ± SEM of three independent experiments. RBL-2H3 cells (1.5 × 10⁶/well) were sensitized with anti-DNP IgE (50 ng/mL). After overnight incubation, the cells were pretreated with or without EL for 1 h and then challenged with DNP-HSA (100 ng/mL). (C) The activation of signaling proteins and NF-κB was assayed by western blot (N, nuclear). b-Actin and lamin B were used as loading controls. The band is a representative of three independent experiments. (D) NF-κB-dependent transcriptional activity was determined by luciferase activity assay. ^∗p < 0.05 compared with the DNP-HSA-challenged group. Dexa, dexamethasone.