Table 3.
Reusability studies of biosilicified lipase in the esterification reactions using ultrasound.
| Conversion alkyl valerate (%) | Specific activity | Protein loading | |||
|---|---|---|---|---|---|
| Alcohols | (U/g biocatalyst) | (%) | |||
| Reuses | Ethanol | 1-Propanol | 1-Butanol | ||
| R0 | 90 ± 4 | 43 ± 6 | 72 ± 3 | 616 ± 9 | 86 |
| R1 | 84 ± 8 | 45 ± 4 | 70 ± 2 | 586 ± 7 | 82 |
| R2 | 78 ± 3 | 40 ± 6 | 76 ± 3 | 524 ± 11 | 70 |
| R3 | 68 ± 6 | 46 ± 2 | 60 ± 6 | 465 ± 8 | 54 |
| R4 | 60 ± 2 | 42 ± 1 | 54 ± 4 | 430 ± 9 | 53 |
Reaction conditions, 0.01 mol valeric acid (1.09 mL), 0.02 mol EtOH/ i-PrOH/ BuOH (1.16 mL, 1.53 mL, 1.83 mL, respectively), 30 mg biosilicified lipase (15% m/v), 2 h ultrasonic irradiation.