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无血清悬浮培养L9981球体细胞和血清贴壁生长L9981细胞在显微镜下形态学差异。A：L9981含血清贴壁培养后形态（100×）；B：L9981无血清培养第3天形态（100×）；C：L9981无血清培养第5天形态（100×）D：L9981无血清培养第6天形态（200 ×）；E：L9981无血清培养第8天形态（100×）（细胞球坏死崩解）；F：L9981无血清培养第10天形态（100×）（细胞球坏死崩解成单细胞状态）。

Morphology of the L9981 spheres cells in serum-free suspension culture is different from the L9981 cells in serum adherent morphological under the microscope. A: the morphology of L9981 cell in serum adherent culture (100 ×); B: the morphol-ogy of L9981 cell in serum-free suspension on the third day (100 ×); C: the morphology of L9981 cell in serum-free suspension on the fifth day (100 ×); D: the morphology of L9981 cell in serum-free suspen-sion on the sixth day (200 ×); E: the morphology of L9981 cell in serum-free suspension on the eighth day (the spheres in necrosis and Collapse) (100 ×); F: the morphology of L9981 cell in serum-free sus-pension On the tenth day ((the spheres in single-cell state) (100 ×).