. 2018 May 20;21(5):365–374. [Article in Chinese] doi: 10.3779/j.issn.1009-3419.2018.05.03

1.

CTC分型鉴定所用探针序列

Probe sequence used in the identification of CTC classification

Gene	Sequences (5’→3’)
CTC: circulating tumor cell.
EpCAM	TGGTGCTCGTTGATGAGTCA AGCCAGCTTTGAGCAAATGA AAAGCCCATCATTGTTCTGG CTCTCATCGCAGTCAGGATC TCCTTGTCTGTTCTTCTGAC CTCAGAGCAGGTTATTTCAG
Vimentin	GAGCGAGAGTGGCAGAGGAC CTTTGTCGTTGGTTAGCTGG CATATTGCTGACGTACGTCA GAGCGCCCCTAAGTTTTTAA AAGATTGCAGGGTGTTTTCG GGCCAATAGTGTCTTGGTAG
CK8	CGTACCTTGTCTATGAAGGA ACTTGGTCTCCAGCATCTTG CCTAAGGTTGTTGATGTAGC CTGAGGAAGTTGATCTCGTC CAGATGTGTCCGAGATCTGG TGACCTCAGCAATGATGCTG
CK18	AGAAAGGACAGGACTCAGGC GAGTGGTGAAGCTCATGCTG TCAGGTCCTCGATGATCTTG CAATCTGCAGAACGATGCGG AAGTCATCAGCAGCAAGACG CTGCAGTCGTGTGATATTGG
CK19	CTGTAGGAAGTCATGGCGAG AAGTCATCTGCAGCCAGACG CTGTTCCGTCTCAAACTTGG TTCTTCTTCAGGTAGGCCAG CTCAGCGTACTGATTTCCTC GTGAACCAGGCTTCAGCATC
Twist	ACAATGACATCTAGGTCTCC CTGGTAGAGGAAGTCGATGT CAACTGTTCAGACTTCTATC CCTCTTGAGAATGCATGCAT TTTCAGTGGCTGATTGGCAC TTACCATGGGTCCTCAATAA
CD45	TCGCAATTCTTATGCGACTC TGTCATGGAGACAGTCATGT GTATTTCCAGCTTCAACTTC CCATCAATATAGCTGGCATT TTGTGCAGCAATGTATTTCC TACTTGAACCATCAGGCATC