1.
构建sgRNA的核苷酸序列
The nucleotide sequence for constructing sgRNA
| sgRNA | The nucleotide sequence (5’----3’) |
| sgRNA-Ezh2-1-oligo1 | CACCG CCCTAGTCCCGCGCAATGAGCTC |
| sgRNA-Ezh2-1-oligo2 | AAAC GAGCTCATTGCGCGGGACTAGGG C |
| sgRNA-Ezh2-2-oligo1 | CACCG CCTGAATGCAGTCGCCTCGGTGC |
| sgRNA-Ezh2-2-oligo2 | AAAC GCACCGAGGCGACTGCATTCAGG C |
| sgRNA-lacZ- oligo1 | CACCG CGATCGTAATCACCCGAGTG |
| sgRNA-lacZ- oligo2 | AAAC CACTCGGGTGATTACGATCG C |