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. 2001 Nov 6;2:8. doi: 10.1186/1471-2164-2-8

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Copyright © 2001 Shackleford et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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Immunoblot analysis of the cellular localization of HA-tagged NPM3. Protein samples (50 mg) were subjected to SDS-PAGE, electrophoretic transfer to nitrocellulose and immunoblotting with an anti-HA antibody. Media, cytoplasm and nucleus are the subcellular fractions analyzed. The locations of the molecular weight standards in kDa are indicated to the left of the blot. Arrows indicate bands associated with HA-tagged NPM3. (A) cells mock-transfected; (B) cells transfected with antisense HA-tagged NPM3 cDNA construct; (C) cells transfected with sense HA-tagged NPM3 cDNA construct.