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. 2018 Jun 13;7:107. doi: 10.1038/s41426-018-0113-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Coomassie Blue staining after SDS-PAGE of macaque serum and purified IgG. M molecular weight markers (kDa). Lane 1: pooled hyperimmune rhesus macaque serum (diluted 500-fold). The filtered start serum mainly contains albumin (arrow). Lane 2: the flow through pool and unbound material (diluted 50-fold). Albumin and other proteins were removed and could be observed in the flow through pool. Lane 3: the effluent and column wash. Lane 4: the eluted and purified IgG (diluted 1000-fold). The IgG heavy chain (~50 kDa) and light chain (~25 kDa) (arrows) could be observed in the pooled elution buffer without albumin and other proteins. b HI and the MN neutralizing antibody titer of the pooled hyperimmune sera and the purified polyclonal IgG antibody. Three recombinant viruses, rH5N1/VN04, rH5N6/GZ14, and rH7N9/AH13, were used to immunize the rhesus macaques. HI titers are presented as the reciprocal value of the highest serum dilution that inhibited hemagglutination. MN titers are presented as the reciprocal value of the highest serum dilution that conferred 50% neutralization of 100 TCID₅₀ of the virus