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. 2014 Jun 20;17(6):469–473. [Article in Chinese] doi: 10.3779/j.issn.1009-3419.2014.06.06

1.

实验所使用的突变引物序列

Oligonucleotides used in the experiments

Site Sequences 5'-3'
The type Ⅱs restriction enzyme site, Esp3I, is underlined. The mutational sites are in red.
Site1A 5'-gatgacgtctcaInline graphicatggccaactgtgagcgtaccttcattgcgatca-3’
Site1B 5'-gcttaaccgtctctcttccttgagctcgagatctgagtccggtag-3’
Site2A 5'-gaacacgtctcaattcInline graphiccttgttggtctgaaattcatgcaagcttccg-3’
Site2B 5'-gataacgtctctgaatcctttctgctcaaaacgcttgataatctctc-3’
Site3A 5'-gaagacgtctcacInline graphicctgcagactccaagcctgggaccatccg-3’
Site3B 5'-gaaatcgtctcaagagagcatgactcggcccgtcttcaccacattcag-3’
Site4A 5'-gcgatcgtctctInline graphictttcaccctgaggaactggtagattacacgagc-3’
Site4B 5'-gtttccgtctccaagccaagccgatctccttctctgcactctc-3’