Figure 2.
Mean proportions of small (0–1 day post hatching; see figure 1), medium (2–3 days) and large (4–5 days) larvae removed from cells in 20 A. mellifera colonies. All larvae were in unsealed cells when treated (day 0). Cell sealing, shown as ‘capped cells’, occurs when a larva is around 6 days old, which would be around 5, 3 and 1 days after treatment for small, medium and large larvae, respectively. Treatments were: CON0, cell monitored but not treated; CONw, 5 µl distilled water placed in base of a cell in larval food at day 0; CHB, 5 µl water suspension of around 5000 chalkbrood, Ascosphaera apis, spores placed in base of cell in larval food at day 0; FKB, larva killed with liquid nitrogen introduced into the cell on day 0.