4.

不同研究者免疫组化法检测EGFR突变灵敏度的总结

Sensitivity of IHC in detecting EGFR mutations from different studies

Studies	n	IHC methodology	Gold standard	IHC scoring criteria	Sensitivity in detecting EGFR mutations by IHC
					Specific Abs in detecting E19/E21 mutations	E746-A750del Ab in detecting E19 mutations	L858R Ab in detecting E21 mutations
TMA: tissue microarray.
Kawahara[29]	60	Slides	DNA sequencing	4 grades, visual scoring	32/44 (72.7%)	13/21 (61.9%)	19/23 (82.6%)
Kato[13]	70	TMA	DNA sequencing	H score, cut off values at 20	18/30 (60.0%)	9/18 (50.0%)	9/12 (75.0%)
Kitamura[15]	238	TMA	DNA sequencing	4 grades, digital scoring	28/78 (35.9%)	16/41 (39.0%)	12/37 (32.4%)
Simonetti[14]	78	Slides	DNA sequencing	4 grades, visual scoring	45/56 (80.4%)	20/29 (69.0%)	25/27 (92.6%)
Ilie [30]	61	TMA	DNA sequencing	4 grades, visual scoring	No mutations in exon 21	9/10 (90.0%)	No mutations in exon 21
Ambrosini-Spaltro[9]	33	Slides	DNA sequencing	4 grades, visual scoring	11/18 (61.1%)	6/12 (50.0%)	5/6 (83.3%)
Hofman[8]	154	Slides	DNA sequencing	4 grades, visual scoring	17/57 (29.8%)	13/36 (36.1%)	4/21 (19.0%)
Jiang[11]	399	Slides	TaqMan PCR	4 grades, visual scoring	118/162 (72.8%)	69/90 (76.7%)	53/76 (69.7%)
Our study	40	Slides	Liquid chip technology	4 grades, visual scoring	16/40 (40.0%)	9/20 (45.0%)	7/20 (35.0%)