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目标基因的引物设计
Primers for two genes
| Gene | Up-primer/ Down-primer | Annealing temperature | Length |
| β-actin | CACTGTGTTGGCGTACAGGT | 62.5 ℃ | 154 bp |
| TCATCACCATTGGCAATGAG | |||
| mTOR | CGCTGTCATCCCTTTATCG | 56.5 ℃ | 193 bp |
| ATGCTCAAACACCTCCACC | |||
| PTEN | ACCAGGACCAGAGGAAACCT | 55 ℃ | 232 bp |
| GCTAGCCTCTGGATTTGACG |
