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. 2010 Apr 20;13(4):311–316. [Article in Chinese] doi: 10.3779/j.issn.1009-3419.2010.04.08

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T1: begin lesion; T2: normal tissue; T3 and T4: lung cancer tissue; P: positive control; N: negative control; U, amplified product with primers recognizing the unmethylated sequences; M: amplified product with primers recognizing the methylated sequences. <break/>Hep-2 DNA methlated with Sss I methyltransferase as a positive control and distilled water was used as a negative control.

肿瘤组织、良性病变及癌旁正常组织甲基化特异性PCR产物电泳结果

Methylation analysis of RASSF1A by MSP in tissue of tumor, benign lesion and normal tissue