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RASSF1A启动子区甲基化特异性PCR扩增引物序列
MSP primer sequence of RASSF1A promter
| M | U | |
| M: methylated DNA specific primer; U: unmethylated DNA specific primer. | ||
| Nest 1 | ||
| Sense primer | 5’ACGAAGGAGGGAAGGAAG3’ | 5’GATGAAGGAGGGAAGGAA3’ |
| Antisense primer | 5’CCCGCAACTCAATAAACT3’ | 5’CTCCCACAACTCAATAAACT3’ |
| Nest 2 | ||
| Sense primer | 5’GGGTTTTGCGAGAGCGCG3’ | 5’GGTTTTGTGAGAGTGTGTTTAG3’ |
| Antisense primer | 5’GCTAACAAACGCGAACCG3’ | 5’CACTAACAAACACAAACCAAAC3’ |
| Products | 169 bp | 169 bp |
