3.
CD36、PMAIP1及FABP4的荧光定量PCR实验结果
Expression of three genes CD36, PMAIP1 and FABP4 using fluorescent quantitation PCR
| Gene | Sample | Average Ct | Average△CT | Average △△CT | 2-△△Ct |
| △Ct=target gene Ct-actin Ct; △△Ct=sample target gene△Ct-reference sample target gene△Ct; when the amplification rate of PCR get close to 100%, relative sample template product=2-△△Ct. | |||||
| CD36 | 16-HBE | 31.41 | 13.43 | 0 | 1 |
| CD36 | A549 | 32.21 | 13.73 | 0.285 | 0.821 |
| PMAIP1 | 16-HBE | 34.34 | 16.36 | 0 | 1 |
| PMAIP1 | A549 | 40.96 | 22.48 | 7.12 | 0.0072 |
| FABP4 | 16-HBE | 31.24 | 13.26 | 0 | 1 |
| FABP4 | A549 | 32.51 | 14.03 | 0.77 | 0.588 |
| β-actin | 16-HBE | 17.98 | |||
| β-actin | A549 | 18.48 | |||