1.

实验所使用的突变引物序列

Oligonucleotides used in the experiments

	Sequence 5'-3'
V-primer A	5' AAGCT CGTCTC TCGCACATATCGAGGTGGACATTAC 3'
V-primer B	5' GGATA CGTCTC TGCGTAGCGCTTCATGGCTTTGTGCA 3'
B-primer A1	5' GGCAT CGTCTC TGTGTAGTGCTGCCATTACCATGAG 3'
B-primer B1	5' CGAAT CGTCTC AACACAATTCTCTTACCGTCATGCCATCCG 3'
B-primer A2	5' AAGCT CGTCTC TACTGTGTTCCATGGTGACTGTAGGTGATGC 3'
B-primer B2	5' GGATA CGTCTC TCAGTGGAATGGTGCCTAAAGCCCTGGTG 3'
Mutagenic fragment A	5' ACGC CCTAGCGCCCGGCATCGCCTCTGCATTGT 3'
Mutagenic fragment B	5' TCGG ACAATGCAGAGGCGATGCCGGGCGCTAGG 3’