2.

ENO1对A549细胞运动和EMT的抑制作用。A：划痕实验：在划痕后第24 h、48 h和72 h连续观察，过表达ENO1的A549细胞划痕恢复能力明显下降（^*t检验，P < 0.05）。图表纵坐标表示划痕宽度相对0 h时划痕宽度比，横坐标表示时间；B：Western blot结果显示，相对于A549-Ctrl，A549-ENO1细胞中，E-cadherin表达明显增加，N-cadherin和Vimentin表达量明显降低；C：梯度TGFβ-1诱导EMT实验：低剂量（0.5 ng/mL）处理A549-Ctrl细胞即可出现大量间质样细胞，而A549-ENO1细胞需要较高剂量（2 ng/mL）处理才能出现一定数量的间质样细胞。

ENO1 inhibits A549 cell mobility and EMT process. A: Wound-healing assay: a continuous observation showed that over-expression of ENO1 resulted in limitation of A549 mobility (^*t-test, P < 0.05). In the chart, Y-axis represents the relative wound width and X-axis represents time period; B: Western blot assay: compared to A549-Ctrl, E-cadherin up-regulated in A549-ENO1, with down-regulation of N-cadherin and Vimentin; C: gradient dose of TGFβ-1 inducing EMT: quantity of mesenchymal-like cells were obtained in A549-Ctrl population after low dose (0.5 ng/mL) of TGFβ-1 inducing. Relative lower ratio of mesenchymal-like cells formed in A549-ENO1 population after high dose (2 ng/mL) of TGFβ-1 treatment.