1.

NSCLC细胞株和患者中TIF1γ的表达。A：实时定量PCR法检测HBE、A549和95C细胞中TIF1γ mRNA水平的表达；B：Western blot检测HBE、A549和95C细胞中TIF1γ蛋白的表达；C：用ImageJ灰度扫描软件计算TIF1γ和GAPDH的表达，并用TIF1γ/GAPDH的值作为TIF1γ的相对表达量；D：实时定量PCR法检测13对非小细胞肺癌组织与癌旁组织中TIF1γ mRNA的表达，每对癌组织与癌旁组织均取自同一个体。

Expression of TIF1γ in NSCLC cell lines and NSCLC patients. A: The mRNA levels of TIF1γ and GAPDH in HBE, A549 and 95C were determined by quantitative RT-PCR; B: Representative Western blot of TIF1γ protein levels in HBE, A549 and 95C NSCLC cells; C: Relative estimation for TIF1γ expression in cell lines. ImageJ was used to evaluate the gray value of TIF1γ and GAPDH, and the ratio of TIF1γ/GAPDH was used as relative expression; D: Reletive expression of TIF1γ mRNA in human NSCLC tissues (T) and their paired normal lung tissue (N), each pair obtained from the same patient. NSCLC: non-small cell lung cancer.