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. 2018 May 31;2018:8953958. doi: 10.1155/2018/8953958

Figure 1.

Figure 1

Effects of crude MCS: neurite outgrowth of PC-12 cells at 7 days grown on collagen-coated plates: controls (top), 7S NGF 0.5 µg/mL (mid), and MCS extract (150µg/mL) (bottom). Fluorescent neurite outgrowth imaging using Molecular Probes® Neurite Outgrowth Staining Kit (A, B, C). Morphology (D, E, F) and changes in neurofilament NF-200 kDa obtained by ICC: primary rabbit anti-rat NF-200 kDa, secondary goat anti-rabbit Alexa 488, and nuclear counterstained with propidium iodide in fixed permeabilized cells (G, H, I) with magnified images (J) control (K) MCS seed.