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Total seed proteins were separated using a gradient gel 8–16% Mini-PROTEAN® TGX™ gel at 200V for 45 minutes. Gels were stained with Blue-band it®, washed in ultrapure water then excised, and electroeluted back into solution at 200V. Samples were reconstituted in HBSS and evaluated for biological activity on PC-12 cells. The 17kDa band contained the main active protein.
