Table 1.
Proteomic analysis of the 17 KD spot. The gel was digested, separated, and evaluated by UPLC-MS/MS using and Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spec. Data were searched using several tandem mass spectrometry protein identification algorithms, including X Tandem and OMSSA/K-score. The data represented protein entry ID, description, E-value, intensity, peptides found, identifications, spectral counts, percent coverage and species, and genus of protein.
| Protein | Description | E-Value | Intensity | Peptides | ID | Spectral Counts | Coverage (%) | Species Genus |
|---|---|---|---|---|---|---|---|---|
| P13744 | 11s globulin subunit beta | -38.9 | 8.53 | 3 | 17 | 12 | 6.67 | Cucumis Sativus |
| A0A0A0LCF7 | Oleosin | -14.7 | 8.48 | 1 | 8 | 8 | 6.96 | Cucumis Sativus |
| Q8L694 | Napin | -37.2 | 8.86 | 3 | 8 | 7 | 17.86 | Momordica charantia |
| P82410 | Two inhibitor peptide topologies 5 | -21.7 | 8.79 | 2 | 4 | 34 | 12.72 | Momordica macrophylla |
| AOAOA7HIA5 | Two inhibitor peptide topologies 6 | -22.3 | 8.72 | 2 | 5 | 4 | 14.16 | Momordica macrophylla |
| P82408 | Trypsin inhibitor 1 MCoTI-1 | -22.3 | 8.72 | 2 | 5 | 4 | 35.29 | Momordica cochinchinensis |
| P82409 | Trypsin inhibitor 2 MCOTI-11 Chain A | -22.3 | 8.72 | 2 | 5 | 4 | 35.29 | Momordica cochinchinensis |
| J3RCD6 | Two inhibitor peptide topologies 1 | -22.3 | 8.72 | 2 | 5 | 4 | 17.08 | Momordica cochinchinensis |
| J7IN40 | Two inhibitor peptide topologies 2 | -22.3 | 8.72 | 2 | 5 | 4 | 17.88 | Momordica cochinchinensis |
| J3R9Z5 | Two inhibitor peptide topologies 3 | -27.3 | 8.72 | 3 | 6 | 4 | 16.71 | Momordica cochinchinensis |
| AOAOAOL2N7 | Non-specific serine/threonine protein kinase | -4.2 | 6.49 | 1 | 1 | 1 | 2.4 | Cucumis Sativus |
| AOAOAOKNN9 | Elongation factor-1 alpha | -2.3 | 6.7 | 1 | 1 | 1 | 2.74 | Cucumis Sativus |