MYL9 expression and S19 phosphorylation in nondiabetic and diabetic gastric fundus and gastric antrum smooth muscles. (a) Representative immunoblots of MYL9 expression in nondiabetic and diabetic fundus (left panel, n = 4, each group) and nondiabetic and diabetic antrum muscles (right panel, n = 4, each group), 500 ng muscle lysate per lane. (b) MYL9 expression relative to enteric γ-actin expression in nondiabetic and diabetic fundus and antrum smooth muscles. (c) Representative immunoblots of phosphorylated S19 in nondiabetic and diabetic fundus (left panel, n = 4, each group) and nondiabetic and diabetic antrum muscles (right panel, n = 4, each group), 1 μg muscle lysate per lane. (d) S19 phosphorylation relative to MYL9 expression in nondiabetic and diabetic fundus and antrum smooth muscles. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.0001.