Table 3.
summary of changes observed in the Tat-secreting strain using model iJV1220
| Subsystem | Tat-secreting vs. Sec-secreting | Tat-secreting vs. Plasmid-bearing | ||||||
|---|---|---|---|---|---|---|---|---|
| N | NAMN | P alt = Tat < Sec | P alt = Tat > Sec | N | NAMN | Palt = Tat < pIJ486 | Palt = Tat > pIJ486 | |
| Amino acid metabolism | 187 | 81 | 0.228 | 0.773 | 187 | 82 | 0.044 | 0.956 |
| Carbon metabolism | 131 | 17 | 0.333 | 0.679 | 131 | 18 | 0.419 | 0.594 |
| Cell envelope | 577 | 292 | 0.000 | 1.000 | 577 | 292 | 0.000 | 1.000 |
| TCA | 17 | 11 | 0.179 | 0.838 | 17 | 10 | 0.153 | 0.864 |
| Cofactor biosynthesis | 214 | 148 | 0.000 | 1.000 | 214 | 148 | 0.000 | 1.000 |
| Exchange | 216 | 43 | 0.646 | 0.357 | 216 | 45 | 0.578 | 0.425 |
| Glycolysis and gluconeogenesis | 21 | 13 | 0.185 | 0.829 | 21 | 12 | 0.397 | 0.625 |
| Inorganic Ion Transport and Metabolism | 59 | 15 | 0.270 | 0.744 | 59 | 15 | 0.230 | 0.782 |
| Nucleotide metabolism | 125 | 47 | 0.023 | 0.978 | 125 | 47 | 0.023 | 0.978 |
| Oxidative Phosphorylation | 17 | 7 | 0.185 | 0.847 | 17 | 6 | 0.188 | 0.852 |
| PPP | 15 | 10 | 0.741 | 0.285 | 15 | 8 | 0.604 | 0.437 |
| Transport, Membrane | 167 | 22 | 0.500 | 0.509 | 167 | 25 | 0.431 | 0.577 |
| Unassigned | 16 | 4 | 0.500 | 0.614 | 16 | 4 | 0.500 | 0.614 |
| Amylase secretion | 5 | 4 | 0.009 | 0.996 | 5 | 0 | NA | NA |
| Agarase secretion | 5 | 4 | 0.996 | 0.009 | 5 | 4 | 0.996 | 0.009 |
Comparisons of the Tat-secreting strain using the model iJV1220 based on MTF analysis of the AMN with pathways grouped in broad subsystems. N is the number of reactions in the subsystem, NAMN is the number of subsystem reactions that are active in either of the two strains being compared, P is the P value obtained using the specified alternative hypothesis (i.e. when P < 0.05 the specified alternative hypothesis cannot be rejected). Only subsystems with NAMN > 4 are reported
Abbreviations: TCA cycle tricaboxylic acid (Krebs) cycle, PPP pentose phosphate pathway, NA not applicable