Table 2.
Comparison of LCM RNA-seq results for endometrial localization of gene expression to results from in situ hybridization and immunohistochemistry
| Gene | LE | GE | S | Complete | Other studies | Technique | PubMed ID |
|---|---|---|---|---|---|---|---|
| AKR1B1 | 5.2 | 5.2 | 2.2 | 5.1 | LE days 12/13 P | ISH | 17640989, 24695626 |
| HPGD | −3.8 | −1.4 | −3.6 | −3.0 | expression in LE D12C | ISH | 24695626 |
| EGFR | −0.7 | d | d | −1.0 | low abundance in LE/GE D12 | ISH | 24012778 |
| FGF7 | 2.3 | 1.7 | d | 2.1 | expression in LE/GE D12P | ISH | 10819782 |
| FGF9 | 2.2 | nd | nd | 3.0 | expression in GE D14P | IHC | 20393170 |
| FGFR2 | d | d | d | d | expression in LE/GE D12 | ISH | 28395342 |
| ABCC1 | 3.5 | d | d | 2.4 | expression in LE/GE D12P (weak) | ISH | 27764917 |
| LOC100738425 (ABCC4) | nd | 2.9 | nd | 2.2 | expression in LE/GE D12P | ISH/IHC | 24695625 |
| ABCC9 | 1.2 | d | d | 2.2 | expression in LE/GE D12P (weak) | ISH | 27764917 |
| SLC24A4 | −8.2 | d | −2.0 | −1.9 | nd in LE D12P | ISH | 24472379 |
| SLCO2A1 | 1.0 | d | 2.3 | d | expression in LE/BV D12P | ISH | 24695625 |
| SLCO4C1 | 1.6 | d | d | 2.3 | nd, ISH too weak, qPCR D12P up | ISH | 27764917 |
| SLCO5A1 | 8.4 | 4.9 | 5.9 | 6.4 | nd, ISH too weak, qPCR only D12 P | ISH | 27764917 |
| GJA1 | 2.0 | d | 1.3 | 1.3 | stroma, BV | IHC | 9669753 |
| GJB1 | nd | nd | nd | −1.3 | nd in LE | IHC | 9669753 |
| GJB2 | nd | nd | nd | nd | nd in LE | IHC | 9669753 |
| IRF2 | 1.3 | d | d | 1.0 | cycle: low all cell types, from D12P up in LE | ISH | 17475929 |
| S100G | 2.9 | d | d | 3.6 | expression in LE D12P | ISH | 19641180 |
| STAT2 | −0.8 | d | d | d | cycle: low in all cell types, after D12P: up in stroma | ISH | 17475929 |
| TRPV6 | 5.6 | 6.3 | 2.1 | 5.9 | strong expression in LE, weak in GE D12P | ISH | 19641180 |
Values are log2 FC; d: expression detected; nd: expression not detectable; LE: luminal epithelium; GE: glandular epithelium; BV: blood vessels; P: pregnant; C: cyclic; ISH: in situ hybridization; IHC: immunohistochemistry