Fig. 4.

Binding of DENV1-immune plasma to recombinant ZIKV and DENV1–4 NS1 proteins. A total of 108 sera (n = 35 for primary infection, n = 20 for secondary infection and n = 53 for unknown) from the DENV1-infected individuals were tested by ELISA for binding to NS1 proteins of ZIKV and DENV1–4 at a 1:100 dilution. Negative controls were plasma from healthy donors (n = 19). Each serum sample was tested in duplicates. Significance was analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test correction