Table 1.
The definitions of behavioural parameters in the novel tank
| Behavioural parameters | Definition |
|---|---|
| Total distance travelled (m) | The total distance in the novel tank |
| Average velocity (cm/s) | The direction and magnitude of zebrafish speed in the novel tank |
| Turn angle (°) | The total turning angle of zebrafish in the novel tank |
| Angular velocity (°/s) | The direction and magnitude of zebrafish angular speed in the novel tank |
| Meandering (°/s) | The degree of turning vs. travel distance |
| Average entry duration in the top (s) | The amount of time spent at the top of the novel tank during each crossing |
| Distance travelled in the top (m) | The total distance moved in the defined top part in the novel tank |
| Time spent in the top (s) | The total time spent in the top part of the novel tank |
| Latency to enter the top (s) | The amount of time to first cross from the bottom part to the top of the novel tank |
| Number of entries to the top | The number of crosses from the bottom part to the top of the novel tank |
| Time spent ratio of top: bottom | The ratio of the time spent on top over bottom |
| Distance travelled ratio of top: bottom | The ratio of the total distance moved in the top part vs. the bottom |
| Entries ratio of top: bottom | The number of crosses from the bottom part to the top of the novel tank |
| Freezing bouts (frequency) | The total number of instances of immobility (> 1 s) during the 5 min test in the novel tank |
| Freezing duration (s) | The duration of all freezing bouts in the novel tank |