Figure 1.

Comparative analysis of Gα_t and Gβ₁ expression levels in retinal lysates from 1-month-old Gγ₁^−/− mice. A, Retinal lysates from Deltagen and StL mouse strains containing 10 μg of total protein were separated by SDS-PAGE alongside serial dilutions of WT retinal lysates and were immunoblotted with antibodies against each transducin subunit. Data are taken from one of three similar experiments. B, The relative contents of Gα_t and Gβ₁ in retinal lysates analyzed in A were determined from the calibration curves obtained from the WT lysate dilutions (Gα_t regression equation: y = 18,600 × x − 623; Gβ₁ regression equation: y = 8130 × x + 665). • = WT, ○ = Deltagen Gγ₁^−/−, and ♢ = StL Gγ₁^−/−. C, The amounts of transducin subunits in Gγ₁^−/− retinas averaged across three independent experiments are expressed as percentages of their amounts in WT retinas (mean ± SD). The amount of Gβ₁ was corrected to reflect that only 18% of Gβ₁ in Gγ₁^−/− retinas is expressed in rods, whereas the rest is expressed in the inner retina (Lobanova et al., 2008).