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. 2018 Jun 11;5(3):ENEURO.0175-18.2018. doi: 10.1523/ENEURO.0175-18.2018

Figure 3.

Figure 3.

Molecular phenotypes of PmCo–AOB projection neurons. A, PmCo corticobulbar projection neurons (ACPs) labeled with Ct-b after AOB retrograde tracing. Ct-b-labeled PmCo neurons (red) express the excitatory markers CaMKIIa (cyan, 92.2%) and Tbr1 (gray, 93.3%). B, C, Ct-b-labeled neurons in the PmCo do not express YFP in GADcre;Ryfp or Dlx5/6cre;Ryfp mouse lines (cyan), confirming their excitatory phenotype. D, PmCo-Ct-b-labeled neurons express Cux1 (cyan, 37.1%) and Ctip2 (gray, 94.5%), typical cortical neuron markers in layer II–IV and V and VI, respectively. All Cux1+ PmCo neurons coexpressed Ctip2. E, Expression of the gene Sim1 reaches ca. 82.4% in PmCo Ct-b-positive cells. For each count, tissue collected (three to four sections) from three Ct-b-injected mice was used. Scale bars: left, 20 μm; right, 200 μm.