Table 1:

Summary of CaMKIIa optogenetic stimulations experiments

AAV expression		Evoked EPCs
PmCo	BAOT	MC	GC	mac
Yes	N/D	No	Yes	No
No	N/D	Yes	N/D
No	N/D	Yes	No
Yes	N/D	Yes	No
Yes	N/D	Yes	Yes	Yes
Yes	No	N/D	Yes
Yes	No	N/D	Yes
Yes	No	N/D	No
Yes	No	N/D	N/D	Yes
Yes	No	No	Yes
Yes	No	No	Yes
Yes	No	No	N/D
Yes	No	No	No
No	No	No	No
Yes	No	No	N/D
No	No	No	No
Yes	No	No	Yes
Yes	No	No	Yes
Yes	No	No	N/D	Yes
Yes	No	No	Yes
Yes	Yes	N/D	N/D
Yes	Yes	Yes	No
Yes	Yes	Yes	N/D
Yes	Yes	Yes	N/D
Yes	Yes	Yes	Yes
Yes	Yes	Yes	N/D	Yes
Yes	Yes	Yes	Yes	Yes
Yes	Yes	Yes	N/D	Yes

Viral delivery of Chr2 to the PmCO was accomplished using a CaMKIIa-specific viral vector (see Materials and Methods) to target excitatory neurons. When the PmCo was efficiently targeted (first left column, PmCo, value = yes), excitatory responses were evoked in AOB GCs. Similar responses were observed also in AOB MCs (MC) but only when the BAOT was labeled (second left column, BAOT, value = yes; N = 7). In other cases (N = 4) MCs were also responsive to perisomatic light stimulations but, due to tissue damage during brain tissue harvest, the area corresponding to the anterior portion of the injection site (in proximity with the BAOT) was not available. This survey was used to assess the likelihood of PmCo-to-GC and BAOT-to-MC connectivity.