Fig. 4.

a CX3CL1 expression in PBMCs from WT and AD BBB models at the ages of 3 months (n = 4 for WT, n = 6 for AD), 6 months (n = 11 for WT, n = 9 for AD), and 12 months (n = 10 for WT, n = 6 for AD). b CX3CL1 expression in luminal lysates from WT and AD BBB models at the ages of 3 months (n = 5 for WT, n = 6 for AD), 6 months (n = 11 for WT, n = 9 for AD), and 12 months (n = 10 for WT, n = 6 for AD). CX3CL1 were analyzed by the 1-plex Luminex® xMAP® assay as described in the “Methods” section. Chemokine levels are expressed in picograms per milligram of protein. The mean is represented by a colored line following origin of cell types (blue for WT and red for AD cells, respectively). a ^*P < 0.05 in PBMCs from WT BBB models at 6 and 12 months compared to WT BBB model at 3 months by Kruskal-Wallis test with a Dunn’s multiple comparison test. ^†P < 0.05 in PBMCs from AD BBB models compared PBMCs from WT BBB model at 3 months by Mann-Whitney’s test. b ^*P < 0.05 in luminal lysates from AD BBB models at 12 months compared to AD BBB models at 3 months by Kruskal-Wallis test with a Dunn’s multiple comparison test. ^†P < 0.05 in luminal lysates from AD BBB model compared to WT BBB model at 12 months by Mann-Whitney’s test