Fig. 3.

No H1975 cell colony survives by co-treatment of IFNγ with Smac mimetic AZD5582. H1975 cells were incubated with 1 or 5 ng/ml IFNγ (IFNγ-1 or IFNγ-5), poly(I:C) (PIC, 250 ng/ml), or poly(I:C) (250 ng/ml) with 1 or 5 ng/ml IFNγ (γ1 + PIC or γ5 + PIC) in the presence of control DMSO or AZD5582 (20 nM) for 4 weeks. Cell colonies were stained with crystal violet shown in plate (a) and crystal violet stain was eluted and quantified at OD540 nm (b). Results represent three independent experiments