Fig. 4.

IFNγ/AZD5582-induced apoptosis is mediated by JAK kinase but not TNFα production in H1975 NSCLC cell line. H1975 cells were incubated with DMSO, AZD5582 (20 nM), IFNγ (10 ng/ml), poly(I:C) (PIC, 250 ng/ml), AZD5582 plus IFNγ (AZD + IFNγ), or AZD5582 plus poly(I:C) (AZD + PIC) in the presence DMSO, Z-VAD-FMK (25 µM), Z-IETD-FMK (IETD-8, 25 µM), necrostatin-1 (Nec-1, 40 µM), JAK inhibitor-1 (500 nM), BAY11-7082 (5 µM), AG1478 (250 nM), TNFα neutralizing antibody (TNFαAb, 1 µg/ml), GSK872 (5 µM), necrosulfonamide (NSA, 1 µM) for 48 h. Cell viabilities were assessed by MTS assay and cell survival rates were calculated by comparison to DMSO-treated control cells (n = 3). ***p < 0.001 versus DMSO. Results represent the findings of three independent experiments